This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.
Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.
Key Features
* Includes a glossary of over 200 terms important for understanding molecular biology
* Uses an inexpensive source of eukaryotic cells – great for schools on a budget
* Includes Methods Locator that provides instant access to the latest methods
* Contain clearly written, easy-to-follow, student-tested instructions:
* Sterile techniques
* Phage titration
* Gel electrophoresis of DNA
* Restriction enzyme digestion
* Plasmid isolation
* Transformation of E. Coli
* Recombinant DNA cloning
* Nick translation labeling
* Nonradioactive primer labelling
* Nonradioactive DNA detection
* Southern blotting
* Colony hybridization
* Purification of plant DNA
* RNA purification
* Northern blotting
* Purification of poly A+ RNA
* Polymerase chain reaction (PCR)
